Journal: Scientific Reports

Article Title: Genome-based reclassification of Micromonospora veneta Kaewkla et al. 2022 as a later heterotypic synonym of Micromonospora coerulea Jensen 1932 (Approved lists 1980)

doi: 10.1038/s41598-025-13676-y

Figure Lengend Snippet: The length of the 16S rRNA gene sequences of M. coerulea JCM 3175 T and M. veneta DSM 109713 T are 1,436 bp and 1,438 bp respectively. Maximum-likelihood tree based on 16S rRNA gene sequences, showing the phylogenetic positions of strains DSM 109713 T and JCM 3175 T and related members within the genus Micromonospora . Actinoplanes aksuensis TRM 88003 T (OM 112204) was used as an outgroup. Bootstrap values (expressed as percentages of 1000 replicates) above 50% are shown at the branch points. Bar, 0.01 substitutions per nucleotide position.

Article Snippet: At the time of writing, based on Parte the genus Micromonospora includes 130 species with validly published names ( https://lpsn.dsmz.de/genus/micromonospora ), widely distributed in various environments, including peat swamp forests root noduless hot spring soils and deep sea environments .

Techniques:

Journal: Scientific Reports

Article Title: Genome-based reclassification of Micromonospora veneta Kaewkla et al. 2022 as a later heterotypic synonym of Micromonospora coerulea Jensen 1932 (Approved lists 1980)

doi: 10.1038/s41598-025-13676-y

Figure Lengend Snippet: Pangenome analysis of the 16 Micromonospora type strains. (A) A pangenome map depicting the functional distribution of core gene clusters and unique genes in the selected Micromonospora genomes. (B) The accumulative curve showing the number of core gene clusters in relation to the number of genomes included in the pangenome analysis. The blue line represents the change in number of core gene clusters as the number of genomes included in the pan-genome analysis increases. The orange line typically indicates the number of non-core gene clusters (or gene clusters, variable gene clusters) as the number of genomes included changes. (C) UpSet plot illustrating the unique genes as well as the genes shared between the Micromonospora strains.

Article Snippet: At the time of writing, based on Parte the genus Micromonospora includes 130 species with validly published names ( https://lpsn.dsmz.de/genus/micromonospora ), widely distributed in various environments, including peat swamp forests root noduless hot spring soils and deep sea environments .

Techniques: Functional Assay

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: HPLC-ELSD chromatogram of the crude extract of Micromonospora sp. 28ISP2-46 T .

Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

Techniques:

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: Phylogenetic tree for strain 28ISP2-46 T and the other group IA Micromonospora species generated using the TYGS and drawn with iTOL. The tree was constructed using FastME 2.1.6.1 from GBDP distances calculated from genome sequences. The branch lengths are scaled in terms of GBDP distance formula d5. The numbers above branches are GBDP pseudo-bootstrap support values from 100 replications, with an average branch support of 98.4% and a delta statistic of 0.135. The tree was rooted using Micromonospora pallida as the outgroup.

Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

Techniques: Generated, Construct

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: ( a ) ClusterBLAST hits for the kosinostatin cluster. Homologues of kst genes are coloured as follows: kstA genes red, kstB genes yellow, kstC genes green, kstD genes blue, kstRg genes black, kstRs genes white, and genes uninvolved in kosinostatin biosynthesis grey. A black line at the end of a cluster indicates that it is close to the end of a contig. Gene sizes not to scale. The NCBI protein IDs for first and last coloured genes of each cluster are as follows: Micromonospora sp. TP-A0468 AFJ52719.1 and AFJ52701.1; Micromonospora sp. 28ISP2-46 T QLQ36639.1 and QLQ36600.1; N. valliformis WP_017579213.1 and WP_017579258.1; N. alkaliphila WP_017604198.1 and WP_017604153.1; Streptomyces sp. SM8 PKA38649.1 and PKA38729.1; Streptomyces sp. ScaeMP-6W SCE31237.1 and SCE31955.1; A. bangkokensis OLR89622.1 and OLR89598.1; M. haikouensis SCF11188.1 and SCF11278.1. ( b ) The same gene clusters rearranged for ease of comparison. Coloured as 6a.

Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

Techniques: Comparison